Human Mesenchymal Stem Cell Culture Protocol

Culturing of mesenchymal stem cells note.

Human mesenchymal stem cell culture protocol. Plate mscs at 1x10 5 cells per well in the fibronectincoated tissue culture plate using msc growth medium 2 work in duplicate. Using a human fibronectin 20 µg ml or a 0 1 gelatin coated 48 well tissue culture plate seed 20k cells well in 0 5 ml normal msc growth media scm015 or scm045 after an overnight incubation replace the culture media with osteomax xf differentiation media. Cryopreserved poietics tm normal human bone marrow derived mesenchymal stem cells hmsc are guaranteed to have at least 0 75 million viable cells per vial differentiate down the adipogenic chondrogenic and osteogenic liniages when cultured in the recommended differentiation medium to express cd29 cd44 cd105 and cd166 and to not express. Coat the culture vessel.

Complete portfolio of primary human mesenchymal stem cells hmsc and optimized growth and differentiation media. Mesenchymal stem cell protocols download a free copy of our gibco mesenchymal stem cell sourcebook for a complete product reference guide product recommendations applications data and publications to support your msc workflow. Coat a 6well tissue culture plate with 10 μg ml human fibronectin or bovine fibronectin according to the instruction manual. Differentiation of human mesenchymal stem cells.

Our hmsc s are available from bone marrow adipose tissue and umbilical cord matrix of individual donors. Seed mesenchymal stem cells. Media and cells we offer everything you need for human mesenchymal stem cell culture. Whether mscs are used in basic research or in translational studies establishing the optimal mesenchymal stem cell expansion method and best performing medium allows.

This procedure uses 20 ml for each t75 flask used. Osteogenesis of mesenchymal stem cells. When handling biohazard materials such as human cells safe laboratory procedures should be followed and protective clothing should be worn.